Synepalum dulcificum

Synepalum dulcificum

Synsepalum dulcificum

Common Names: Miracle fruit, Miracle berry, Sweet berry
Local Names: Agbayun (Yoruba, Nigeria), Azimomo (Edo, Nigeria), Uni (Igbo, Nigeria)
Species ID: NMP-251 |
NCBI_Taxonomy ID: 3743 | wfo-0000310701

Scientific Classification

Kingdom: Plantae

Clade: Tracheophytes

Clade: Angiosperms

Clade: Eudicots

Clade: Asterids

Order: Ericales

Family: Sapotaceae

Genus: Synsepalum

Species: S. dulcificum

Synonyms: Bakeriella dulcifica (Schumach. & Thonn.) Dubard, Bumelia dulcifica Schumach. & Thonn., Pouteria dulcifica (Schumach. & Thonn.) Baehni, Richardella dulcifica (Schumach. & Thonn.) Baehni, Sideroxylon dulcificum (Schumach. & Thonn.) A.DC., Synsepalum glycydora Wernham

Morphological Description

Synsepalum dulcificum is a small, evergreen understory shrub or tree, typically 2–4 meters tall, occasionally reaching 6 meters in native habitats. Its key characteristics include:

·       Bark: Smooth, greyish-brown, with small lenticels; used in traditional medicine (Burkill, 1995).

·       Leaves: Simple, alternate, obovate to elliptic (5–10 cm × 2–4 cm), glossy dark green, clustered at branch tips; petioles short (0.5–1 cm).

·       Flowers: Small (5–7 mm), white to cream, solitary or in pairs in leaf axils; 5-petaled, fragrant, blooming year-round in tropical climates (Prance & Jongkind, 2015).

·       Fruit: Oblong, red berries (2–3 cm × 1 cm), with thin pulp surrounding a single seed; contains miraculin, a taste-modifying glycoprotein (Akinmoladun et al., 2020).

Distribution and Habitat

Synsepalum dulcificum is native to West and Central Africa, with cultivation expanding globally:

·       Geographic Range: Native to Nigeria, Ghana, Benin, Cameroon, DR Congo, and Togo; introduced to tropical regions like Malaysia, India, and Australia; cultivated in Nigeria for medicinal and culinary uses (Kew Science, n.d.).

·       Habitat: Thrives in lowland rainforests, secondary forests, and along watercourses; prefers moist, well-drained, loamy soils (pH 4.5–6.0) with annual rainfall of 1,200–2,500 mm; occurs at elevations up to 400 m.

·       Ecological Role: Supports pollinators like bees; fruits are consumed by birds, aiding seed dispersal; enhances understory biodiversity (Burkill, 1995).

Ethnopharmacology

Synsepalum dulcificum is widely used in West African traditional medicine, with extensive ethnopharmacological validation for multiple ailments (Akinmoladun et al., 2020):

·       Antidiabetic: In Nigeria, leaf decoctions treat diabetes. Methanolic leaf extracts (30–60 mg/kg) reduced blood glucose by 40% in fructose-fed streptozotocin-induced diabetic rats, enhancing insulin secretion and inhibiting α-amylase (IC50: 0.5 mg/mL) (Obafemi et al., 2017).

·       Antioxidant: In Ghana, fruits combat oxidative stress. Methanolic fruit extracts showed DPPH scavenging (60.05% at 1 mg/mL) and high phenolic content (625.57 mg GAE/100 g), reducing ROS in biological systems (Babatunde et al., 2019).

·       Anticancer: In Nigeria, fruit extracts are used for tumors. Ethanolic fruit extracts exhibited cytotoxicity against HCT-116 colorectal cancer cells (IC50: 8.54–14.99 μg/mL), with minimal toxicity to normal cells (IC50: 196.72–236.25 μg/mL) (Afzal et al., 2021).

·       Anti-hyperlipidemic: In Cameroon, leaves lower cholesterol. Methanolic leaf extracts (200 mg/kg) reduced LDL cholesterol by 30% in hyperlipidemic rats, attributed to flavonoids like catechin (Xie et al., 2024).

·       Antimalarial: In Benin, leaves treat malaria. Ethanolic leaf extracts showed moderate antiplasmodial activity, supporting traditional use, though specific compounds were not isolated (Okokon et al., 2008).

·       Antimicrobial: In Nigeria, fruit extracts treat infections. Petroleum ether fruit extracts inhibited Bacillus subtilis and Staphylococcus aureus (MIC: 50–100 μg/mL), linked to essential oils (Afzal et al., 2021).

·       Taste Modification: In Nigeria, berries are used to sweeten sour foods. Miraculin binds to sweet taste receptors under acidic conditions, converting sour to sweet tastes, aiding chemotherapy patients with taste disorders (Swamy et al., 2014).

⚠  Toxicity Profile: Synsepalum dulcificum is generally safe at therapeutic doses. Sub-acute administration of leaf extracts up to 200 mg/kg showed no toxicity in rats, with no significant hematological or biochemical changes. Mild side effects, such as stomach ache and throat discomfort, were reported in some human studies. High doses (>1,000 mg/kg) may cause liver enzyme elevation (ALT increased by 10% after 30 days). Use is contraindicated in pregnancy and for children due to insufficient safety data. Potential heavy metal contamination in wild-harvested plants requires caution (Akinmoladun et al., 2020; Swamy et al., 2014).

Phytochemistry

The bioactivity of Synsepalum dulcificum is attributed to its diverse phytochemical profile (Akinmoladun et al., 2020):

·       Glycoproteins: Miraculin (0.1–0.5% in fruit pulp), responsible for taste modification (Swamy et al., 2014).

·       Flavonoids: Quercetin, catechin, kaempferol, with antioxidant and antidiabetic effects (Obafemi et al., 2017).

·       Phenolic Acids: Gallic acid, chlorogenic acid, enhancing antimicrobial and anti-hyperlipidemic properties (Xie et al., 2024).

·       Alkaloids: Dihydro-feruloyl-5-methoxytyramine, N-cis-caffeoyltyramine, supporting antimalarial activity (Akinmoladun et al., 2020).

·       Lignins: (+)-Syringaresinol, (+)-epi-syringaresinol, with anticancer potential (Afzal et al., 2021).

Additional Uses

·       Culinary: Berries are used in Nigeria to sweeten sour foods like palm wine and citrus; popular in “flavor-tripping” events globally.

·       Ornamental: Cultivated in Ghana for its attractive foliage and red berries, enhancing garden aesthetics (Burkill, 1995).

·       Nutritional: Fruits contain vitamin C (40.1 mg/100 g), low sugars (5.6 g/100 g), and high antioxidant capacity (457.3 μmol Trolox/100 g) (Inglett & Chen, 2011).

·       Industrial: Seed oil, rich in oleic and palmitic acids, is explored for cosmetic and pharmaceutical applications (Babatunde et al., 2019).

References

  • Afzal, S., Raju, A. V., Raju, C. S., Chong, G., Wuii, Z., & Eseyin, O. A. (2021). Evaluation of the antimicrobial and anticancer properties of the fruits of Synsepalum dulcificum (Sapotaceae). Tropical Journal of Pharmaceutical Research, 20(9), 1925-1930.
  • Akinmoladun, A. C., Adetuyi, A. R., Komolafe, K., & Oguntibeju, O. O. (2020). Nutritional benefits, phytochemical constituents, ethnomedicinal uses and biological properties of Miracle fruit plant (Synsepalum dulcificum Shumach. & Thonn. Daniell). Heliyon, 6(12), e05837.
  • Babatunde, O., Oladimeji, A. O., & Oguntuase, B. J. (2019). Chemical composition and antioxidant properties of Synsepalum dulcificum Daniell and Carica papaya L. seeds oil. Journal of Chemical Society of Nigeria, 44(7), 1234–1240.
  • Burkill, H. M. (1995). The useful plants of West Tropical Africa (Vol. 3). Royal Botanic Gardens, Kew.
  • Inglett, G. E., & Chen, D. (2011). Contents of phenolics and flavonoids and antioxidant activities in skin, pulp, and seeds of miracle fruit. Journal of Food Science, 76(3), C479–C482.
  • Kew Science. (n.d.). Synsepalum dulcificum (Schumach. & Thonn.) Daniell. Plants of the World Online. https://powo.science.kew.org/taxon/urn:lsid:ipni.org:names:789924-1
  • Obafemi, T. O., Akinmoladun, A. C., Olaleye, M. T., Agboade, S. O., & Onasanya, A. A. (2017). Antidiabetic potential of methanolic and flavonoid-rich leaf extracts of Synsepalum dulcificum in type 2 diabetic rats. Journal of Ayurveda and Integrative Medicine, 8(4), 238–246.
  • Okokon, J. E., Ettebong, E., & Antia, B. S. (2008). In vivo antimalarial activity of ethanolic leaf extract of Stachytarpheta cayennensisIndian Journal of Pharmacology, 40(3), 111–113.
  • Oladosu, P. O., Egharevba, H. O., Okhale, S. E., & Okogun, J. I. (2013). Phytochemical screening, antimalarial and histopathological studies of Allophylus africanus and Tragia benthamiiJournal of Pharmaceutical and Biomedical Sciences, 29(29), 684–689.
  • Prance, G. T., & Jongkind, C. C. H. (2015). Sapotaceae. In Flora of West Tropical Africa. Royal Botanic Gardens, Kew.
  • Swamy, K. B., Hadi, S. A., Sekaran, M., & Pichika, M. R. (2014). The clinical effects of Synsepalum dulcificum: A review. Journal of Medicinal Food, 17(11), 1165–1169.
  • Xie, J., Chen, X., Tan, Y., Li, W., Yin, C., Zhong, T., ... & Huang, W. (2024). Anti-hyperlipidemic components of the leaves of Synsepalum dulcificum (Miracle Fruit). Plant Foods for Human Nutrition, 79(2), 451–459.

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Compounds of Synepalum dulcificum
References


Busari, A. A., Ikumawoyi, V. O., Yusuff, O. S., Adeosun, A. R., Sowemimo, A. A., & Awodele, O. (2022). Ethanol Leaf Extract of Synsepalum dulcificum Schumach. & Thonn.(Sapotaceae) Induces Distortions in Renal, Hepatic and Reproductive Indices in Sprague-Dawley Rats. Pharmacology and Toxicology of Natural Medicines (ISSN: 2756-6838), 2(2), 50-65.